Pyridine Nucleotide Synthesis

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Pyridine nucleotide synthesis

The synthesis of NAD and NADP by rat adipose tissue was measured in vitro. Nicotinamide-7-14C and NaHPP04 were incorporated together into NAD with a =P/14C ratio of 1.82 and nicotinic-7-14C acid and NaH232POa with a ratio of 1.94. Nicotinic acid stimulated, by 90%, lipogenesis from glucose-UW by rat adipose tissue in vitro. Glucose plus insulin and refeeding for 48 hr after a 48 hr fast markedl...

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Effect of hyperthyroidism on liver pyridine nucleotide synthesis.

The concentration of pyridine nucleotides in the liver of the hyperthyroid rat is low (14). Whether this is a result of decreased synthesis, increased destruction, or both, is not known. Several groups of investigators have shown that when the normal rat or the normal mouse is given a single dose of nicotinamide the concentration of pyridine nucleotides in the liver rises rapidly for a few hour...

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Pyridine Nucleotide Transhydrogenase

established unequivocally that either a hydrogen or electron transfer was involved in this reaction. The possibility of a transfer of the monoester phosphate group or an exchange of reduced and oxidized nicotinamidecontaining moieties (nucleoside, nucleotide, etc.) was ruled out by this and other experiments. There still remained, however, the problem of distinguishing experimentally between th...

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A reduced pyridine nucleotide pyrophosphatase.

Reaction 1 is catalyzed by DPNase and has been observed in higher animals (1, 2), in Neurospora (3), and in some bacteria (4). Reaction 2 is catalyzed by a deaminase found in Taka-Diastase (5). Reaction 3 is the DPN pyrophosphorylase from hog liver, as described by Kornberg (6) ; it is the only reversible reaction of those shown. The pyrophosphatase splitting shown in Reaction 4 has been observ...

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Studies on pyridine nucleotide biosynthesis.

Until recently it had been generally accepted that the formation of diphosphopyridine nucleotide in biological systems starts from nicotinamide, with nicotinamide riboside and nicotinamide mononucleotide as intermediates (Singer & Kearney, 1954). Much of the earlier work on whether nicotinic acid or nicotinamide is the more efficient precursor of diphosphopyridine nucleotide has been controvers...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1972

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(20)81802-x